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Image Search Results
Journal: The Kaohsiung Journal of Medical Sciences
Article Title: Exosomal microRNA ‐15a from ACHN cells aggravates clear cell renal cell carcinoma via the BTG2 / PI3K / AKT axis
doi: 10.1002/kjm2.12428
Figure Lengend Snippet: miR‐15a targets BTG2 in ccRCC cells. (A) Targeting relation between miR‐15a and BTG2 verified by dual‐luciferase reporter gene assay (* p < 0.05; compared using two‐way ANOVA). (B) BTG2 expression in UMRC‐2 cells treated with exosomes determined by RT‐qPCR (* p < 0.05; compared using unpaired t test). (C) BTG2 expression in ccRCC tissues and adjacent normal tissues detected by RT‐qPCR (* p < 0.05; compared using paired t test). (D) Correlation analysis between miR‐15a and BTG2 in ccRCC tissues. (E) BTG2 expression in ccRCC cells and HK‐2 cells detected by RT‐qPCR (* p < 0.05; compared using one‐way ANOVA). (F) BTG2 expression quantified by RT‐qPCR following altered expression of miR‐15a (* p < 0.05, ** p < 0.01; compared using one‐way ANOVA). (G) Transfection efficiency of BTG2‐OE in cells verified by RT‐qPCR (* p < 0.05; compared using unpaired t test). (H) Effects of BTG2 on ccRCC cell proliferation determined by colony formation assay (* p < 0.05; compared using unpaired t test)
Article Snippet: The first five nucleotides complementary to the seed region of miR‐15a were mutated by site‐directed mutagenesis, and the
Techniques: Luciferase, Reporter Gene Assay, Expressing, Quantitative RT-PCR, Transfection, Colony Assay
Journal: The Kaohsiung Journal of Medical Sciences
Article Title: Exosomal microRNA ‐15a from ACHN cells aggravates clear cell renal cell carcinoma via the BTG2 / PI3K / AKT axis
doi: 10.1002/kjm2.12428
Figure Lengend Snippet: BTG2 can offset the effects of miR‐15a on ccRCC cells. (A) Establishment of cells overexpressing both miR‐15a and BTG2 verified by RT‐qPCR (* p < 0.05; compared using unpaired t test). (B) Cell proliferation detected by colony formation assay (* p < 0.05; compared using unpaired t test). (C) Cell migration examined by transwell assay (* p < 0.05; compared using unpaired t test). (D) Cell invasion evaluated by transwell assay (* p < 0.05; compared using unpaired t test). (E) Cell apoptosis determined by flow cytometry (* p < 0.05; compared using unpaired t test). (F) EMT activity of cells shown by detecting the florescence intensity of E‐cadherin
Article Snippet: The first five nucleotides complementary to the seed region of miR‐15a were mutated by site‐directed mutagenesis, and the
Techniques: Quantitative RT-PCR, Colony Assay, Migration, Transwell Assay, Flow Cytometry, Activity Assay
Journal: The Kaohsiung Journal of Medical Sciences
Article Title: Exosomal microRNA ‐15a from ACHN cells aggravates clear cell renal cell carcinoma via the BTG2 / PI3K / AKT axis
doi: 10.1002/kjm2.12428
Figure Lengend Snippet: miR‐15a‐mediated BTG2 inhibition increases activity of the PI3K/AKT pathway. Changes of PI3K/AKT pathway in exosomes‐treated cells or cells overexpressing BTG2 or/and miR‐15a detected by western blot analysis
Article Snippet: The first five nucleotides complementary to the seed region of miR‐15a were mutated by site‐directed mutagenesis, and the
Techniques: Inhibition, Activity Assay, Western Blot